Outer wells aligned for proper viewing with FLIPRIdeal for miniaturized 384 well fluorescent homogeneous assaysNot treated (or medium binding)… Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. For nearly 30 years, Molecular Devices has provided scientists with . 384-well TC plate, Poly-d-Lysine, Flat Clear. SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903) Purified luciferase, Promega (Cat# E1701). That explains the lower values on the LmaxII384 plate reader. Plate Formats: 6- to 384-well plates, NanoQuant Plate (Sold Separately) Detection Modes: Luminescence, Fluorescence, Absorbance. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 384 Well Optically Clear Tissue Culture Plate: Plate length: 127. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. With optimized reagents, option to operate the readers. • Easy and gentle immobilization of biotin. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. MultiWash+ Microplate Washer. Extended linear dynamic range. Thermo Scientific Pierce Streptavidin Coated Plates are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays. For more sample throughput, the system canSpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. Well scans from the Gemini XPS Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM /Ethidium Homodimer-1) assay kit. Both COC microplates, the μCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. 96- and 384-well plates; Wavelength(s): 635 nm (classification), 532 nm (reporter) Citations:. The FilterMax F3 can read 96-well and 384-well plates. 6% for the on-board optics (monochromators). Instrument settings are listed in Table 1. Dynamic range > 6 decades . The eight-channel system delivers both high precision and speed when reading 96- and 384-well microplates for endpoint measurements, kinetic assays, and spectral scans. 384 Well Plates; Wavelength(s): 200 to 1000 nm (Abs), 250 - 850 nm (Fluor) Specifications/Features: Shaking, Temperature control, Cuvette port; Read More. #3-000-510)Page 154: Ordering Parts And Accessories. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. 96-, 384-, and 1536-well microplates. 40266. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. The StakMax® Microplate Handling System is a powerful, compact, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers for improved process flow and higher throughput. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. Outer wells aligned for proper viewing with FLIPRIdeal for miniaturized 384 well fluorescent homogeneous assaysNot treated (or medium binding)…Elisa Plate Readers and Microplate Readers at Biocompare. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. Two different agonists, acetylcholine and carbachol, were tested. #ABS Plus). For more sample throughput, the system canSpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in the plate drawer. 0. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Learn how the PathCheck feature can be applied to protein quantitation experiments using Molecular Devices absorbance-based microplate readers. Prepare human iPSC-derived neuronal progenitor cells (NPCs) according to Shi et al. The drawing shows a standard microplate of 15 mm height covered with a lid. Description. molecular interaction studies, and nucleic acid quantification, as well. The narrow bandwidths used by the model increase measurement accuracy and linearity over a wide. #R8190 (10) vials of component A*Instruments with a 96- or 384-well format will accommodate the most commonly used plates. 76 L x 85. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. Plus 384. In the M2, M5 and Plus. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices'. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set ofThe plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). 0 and dispense 10 μL to all wells of the 384-well plate(s) (see Note 13). 384 well polystyrene microplates have plate dimensions of 127. FlexStation® 3 Multi-Mode MicroplateAlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. 1. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader Multimode Readers Comparison Chart. begin reading microplates with seven mouse clicks. It features a modular design that allows users to add or change detection cartridges according to their needs. External Dimensions without lid (mm). Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. tools to expand the boundaries of their research. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Microplates, cuvettes (via adapter) Detectors. 4 decades . book Page 1 Wednesday, October 12, 2011 4:56 PMSpectraMax Plus 384 Microplate Reader (Molecular Devices cat. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. within each well. The FLIPR 384 system comes complete with 384. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. Nanoracks’ Plate-Reader-2 (2nd generation) is a reconfigured Molecular Devices SpectraMax M5e – one of the most reliable, durable, feature-rich microplate readers on the market. 1. Alpha 384 HTS 96- and 384-well . For more sample throughput, the system can384 Well Plate, supplied by Molecular Devices LLC, used in various techniques. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Round well design with a clear flat bottom 2. A control well was set up to verify. 6-, 12-, 24-, 48-, 96- and 384-well plates: Tablet PC: Windows® 8. Like conventional microplate readers, multimode microplate readers detect reaction signals from microtiter plates in order to perform assays such as. For biotherapeutic development, the DynaPro Plate Reader is a comprehensive solution for stability evaluation. 0 out of 5 $ 8,495. The Embla 96/384 Well Washer accepts 96-and 384-well format microplates and is ideal. Cell viability는 세포막 Integrity 또는. All microplate washers enable users to program tank selection, volumes, step times, and cycles for customized procedures. The default pipettor dispensing speed is 50 µL/sec for 96-well plates and 20 µL/ sec for 384-well plates. M2. Filter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. 25mm: Plate width: 85. Multichannel processes: Costar 96-well (3300) and Greiner 384-well (781182/781185) microplates are validated for multichannel processes. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate: 0517 Lit. Molecular Devices Gemini XS Microplate Spectrofluorometer Details. The user-friendly MultiWash™+ Microplate Washer from Molecular Devices, one of the pioneers in microplate detection, includes 20 preset protocols, works with both 96- and 384-well plates, and comes with 4 different wash/rinse bottles for out-of-the-box use. plated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. Temperature control (RT+3°C – 42°C) Liquid dispensers with reagent heater and stirrer. This application is highlighted by the example of a fully automated kinetic solubility screen in 384-well plate format. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. The washer is engineered to be simple to maintain and use, including features that reduce clogging and noise. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Loading Buffer was also added to a set of cell-free wells for background controls. flat-bottom: 50-60 µl. 1 or 10 Tablet PC with USB connection to instrument: Read Speed: 96-well plate: less than 1 minute; 384-well plate: less than 3 minutes: Automation Compatibility: Hardware and software accessible to third-party hardware/software control, including LIMS. Select Start>Control panel>Device Manager. FLIPR Calcium 4 Assay Kit. FlexIII. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. Fluorescent Polarization: Solid black plates, as above. This chapter describes assays where data are recorded using a plate-reader; it does not cover assay methods designed for flow cytometry or high content imaging. 과 같은 Parameter를 검사하여 평가할 수 있습니다. Figure 2-23: Bottle holder To install the bottle holder: 1. 384-well microplate compatible Well read area range: H7 to K22 Figure 1-3 24-Well Micro-Volume Sample Slide Figure 1-4. The Standard Thermo Scientific 384-well plate, Barcoded, White, is compatible with the majority of 384 block PCR and sequencing instruments. com Check our website for a current listing of worldwide distributors. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Products : Ionworks HT, Ionworks Quattro. Option 1: Use a plate. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Recertification service. Temperature Control: ambient from +4 to 45C. firefly luciferase in 96- and 384-well top read Dynamic range: > 6 decades Cross-talk: < 0. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Ideal for miniaturized 384 well fluorescent. minutes and begin reading microplates with seven mouse clicks. After reading the ELISA plate on both readers, each standard curve was nearly identical. The estimated lower limit of detection (LLD) in both 96- and 384-well plates is < 2 fg/well for firefly luciferase in the LMax II 384 and Analyst GT and 2–3 fg/ well in the SpectraMax M5. 96-, 384-, and 1536. Figure 2. Gemini EM Reader scans. SpectraDrop Micro-Volume Microplate (Molecular Devices cat. In addition, SoftMax Pro SoftwareThe Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. 96-384. FLIPR Tetra systems are fluorescence- and luminescence-based microplate readers with an integrated 1536-, 384- or 96-pipettor. X. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. #4TI-0655) Microplates. Versa Max. Plate dimensions: X6001, X6004, X6011, X7001, X7007, X7020, X7022, X5101, X6026. Use the provided 3. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Or scan up to 384 wells at any time to determine the best assay conditions. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. 8 mm distance between well bottoms to plate bottom; 50μL total well volume, with recommended working volume of 5-40μL; High base plate design is compatible with automation and for bar coding; Validated by Molecular Devices for the FLIPR reader. 305 reference level saturation. com. Artel MVS Bundle DM Std/800TSNB Reader Details. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. the industry-leading microplate reader software for the SpectraMax Plus 384 system, allows complete customization of data collection and analysis. It. Technical specifications. VIS absorbance detection. x. Contact; Bioz Stars; Bioz vStars; 86 : Buy from Supplier : Structured Review. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. of samples in both 96- and 384-well microplates or in a cuvette at a selected wavelength for a single point in time (endpoint), over a specified period of time (kinetic), or over a selected wavelength range (spectral scan. #0200-6263) UltraVision™ Plate 384 (Brooks Life Sciences cat. 3% in white 96- and 384-well microplates < 3 x 10-5 (white plates) < 3 x. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. Another consideration, says Amouretti, is plate format. Molecular Devices deliver high throughput while showing good correlation with manual patch clamping data. San Jose, CA 95134. 2. SpectraMax ABS Plus reader utilizes Molecular Devices PathCheck Sensor- the only patented. [Carbachol] (nM) RFU Max. 1 a. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. White plates reflect the light, enabling even low levels of Luminescence to be detected. x. Streamline GLP/GMP compliance. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. For fluorescence intensity assays, the SpectraMax® M2 and M2e units feature an. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. EMax® Plus Microplate Reader, Molecular Devices Catalog Number: (10119-370) Supplier: Molecular Devices Description: Robust, high value microplate reader designed to. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. Figure 1. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. x. use as little as 2 µL samples with 24- or 64-well plates. ) 45 (W) x 70 (H) x 27 (D) Dimensions (cm) 114 (W) x 178. 303 unable to cal dark current. Both 96- and 384-well assay formats yielded similar linearity and dynamic range, indicating the suitability of the dual-luciferase assay and SpectraMax i3x system for both formats. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. To prevent evaporation, the microplate was covered with a qPCR seal. This kit is designed for customers who. 3. 5in (46. M5. Expand the Microplates tree. • The FlexStation™ II³⁸⁴ instrument is designed for 96- and 384-well microplates. Turn on Plate Reader and wait for it to complete its start-up routine. 100 µL (96-well assay) or 25 µL (384-well assay) of CellTiter-Glo reagent was added to each well. 616, 665 nm EM filters 0200-7011. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. M5. This can increase throughput and efficiency, thus saving time and space in the lab. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. 00. 100 µL (96-well assay) or 25 µL (384-well assay) of CellTiter-Glo reagent was added to each well. A 96- and 384-well automated washer. 6-384 (96- and 384-well with pipetting) 96-well microplates. 1. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Integrated stacker option. Inquiry Basket. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. Results Microplate readers read biological, chemical or physical events that occur on a microtiter plate. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. Get Quote . 384-well SBS microplates are designed with corners cut at a1 and h1 positions to conform to SBS nomenclature. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. Molecular Devices Corporation (the “Seller”) shall not be liable for any incidental or consequential damages. The SpectraMax 190 reader can be integrated with Molecular . The FLIPR Fluorometric Imaging Plate Reader system addresses the industry's requirements for higher throughput, minimized sample volume, and cost reduction. Read one sample or up to 384 in a single plate eead using any standard cuvette, or 96-or 384-well microplate. 384- or 96-well read plate containing cells loaded. Microplate readers are commonly used in life science laboratories. 7. x. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. These lab workhorses are used in drug discovery, research, bioassay validation, and. 1. and 25 μL of each concentration was added in triplicate to a 384-well solid white plate. ‘StimB’ is Stimulation Buffer, included in the IP-One Gq kit. After reading the ELISA plate on the EMax® Plus Microplate Reader, the R2 value was 0. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. Plate formats. Plate reader instructions Computer. Microplates for applications that require high sample recovery rates. 384-Well Fully Skirted Plates, Barcoded, including Armadillo 384-Well PCR plates. –Min. #2704174, 2705402, 2705412, 2704180; Drummond Digital Microdispenser cat. Tb. 5–25 µl. The Raman plate reader was designed to simultaneously observe the Raman spectrum of 192 analytes loaded in 192 wells on a standard 384-well plate. Four wash/rinse bottles are included and configurable for both 96- and 384. Figure 6. 0 (or higher) Software; Methods Data acquisition using the Workflow Editor. Absorbance equation. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. • 11/10 • Printed in USA • PN: 0120-1525. 96 deep well plate: packed in sleeves of 6 , 24 per case. In many cases these plates have the same dimensions, check the plate dimensions are the same as one of the plates listed above. Devices’ StakMax® Microplate Stacker in a matter of minutes and . and 384-well microplates at a selected wavelength for a single point in time (endpoint), over a specified period of time (kinetic), or over a selected wave-length range (spectral scan). Instruments with a 96- or 384-well format will accommodate the most commonly used plates. The Standard Thermo Scientific 384-well plate, white, is compatible with the majority of 384 block PCR and sequencing instruments. 020 fmol 0. Values are averages from 5 clean plates, each filled with 200 µL water/well. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. It is more inert and reduces compound interference. All stand-alone instrument functions can be controlled using the software. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. 100 fmol 384-well (75 µL) 0. Turn the reader on and secure the USB cable. SpectraMax ID5 Plate Reader (Molecular Devices). Instrument pricing. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate despite. smartinject technology, atp a, spectramax i3x multi-mode microplate reader, luminescent cell vi, spectramax i3x multi mode microplate reader, spectramax i3x. Outer wells aligned for proper viewing with FLIPR; Ideal for miniaturized 384 well fluorescent. ability to use as little as 2˜µL samples with 24- or 64-well plates. Microplate format. formats from 6- to 384-wells, the SpectraMax iD3 reader is the complete solution for all your research needs. Find our technical support information along with contact details. Integrated stacker option. Embla washers are durable and easy to maintain. the iD5 reader’s filter system. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 96-Deep Round or Square Well Plates. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 384-Well Microarray/High Sample Recovery Plate. Molecular Devices has provided scientists with tools to expand the boundaries of their research. The 384-well plate is limited to a maximum volume of a 30 µl addition in a much smaller diameter well, and using the 96. 003 fmol 0. The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-wellThe FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. +1 800. Analyst, M5, Flex 3, and SpectraMax L readers work differently. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. A snapshot of the Settings screen is shown in Figure 2. #ABS Plus) 4s3 ™ Semi-Automatic Sheet Heat Sealer (Brooks Life Sciences cat. This integrated system provides users with a multi-detection for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. High base plate design is compatible with automation and for bar coding. SpectraDrop High-Throughput Screening (HTS) Kit. Selects either 96-well or 384-well plate for a stand-alone read. 96- and 384-well. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modes <2 fg/well for firefly luciferase in 96- and 384- well top read. Time-resolved phosphorescence. Microarray scanners for one-fluor to multi-fluor [email protected] and Download Molecular Devices SPECTRAmax PLUS 384 operator's manual online. Dual injectors for kinetic assays. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. 96/384 - For the SpectraMax 340PC384, press to select to read a 96-well plate or a 384- well plate. 0 mP 96-well. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. " A versatile UV/VIS spectrophotometer for both 96- and 384-well. ,LTD (CAT#: STEM-S. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. 384-well format. The FLIPR Fluorometric Imaging Plate Reader system addresses the industry's requirements for higher throughput, minimized sample volume, and cost reduction. Microplates for applications that require high sample recovery rates. The results above revealed excellent detection limits and dynamic range in all three Molecular Devices microplate luminometers. Page 154: Ordering Parts And Accessories. QC tools and IQOQ services. formats from 6- to 384-wells, the SpectraMax iD3 reader is the complete solution for all your research needs. Molecular Devices 大中华区 Email: info. F3/F5 Multi-Mode Microplate ReadersBoth reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. Outer wells aligned for proper viewing with FLIPR. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate. FlexIII. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates andCompany Molecular Devices, LLC; Price Get Quote; Catalog Number CSI / CSI FL; Type Cell Imaging System; Measuring Time White light - 96-well/ 384 well microplate: <2 min Fluorescence - 96-well/ 384 well microplate: <8 min; Plate Formats 6, 12, 24, 96 & 384-well SBS microplates; Applications Cell Line Development, Fluorescence, Time-LapseNext-generation luminescence plate reader. 96 Well Plate Reader found in: Well Plate Reader Accessory, SmartReader™ 96 Microplate Absorbance Reader, SmartReader UV-Vis Microplate Reader, Stratus. x. Tecan Infinite 200 Pro M Plex Microplate Reader Unit2 - AV. , San Diego, CA, USA) such that the. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. Plate readers avoiding cross-talk are recommended for luminescence measurement in 384 well plates. 384-WELL 10MM-HEIGHT HIGH SAMPLE RECOVERY MICROPLATES WI/WO LID. #I3X), equipped with the following: HTRF Detection Cartridge (Molecular Devices cat. As the wells of this plate are closer together, the light is more likely to stray into adjacent wells. Microplate Formats: 6, 12, 24, 48, 96, 384. UV absorbance detection. SpectraMax M Series Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. 96-well fluorescent cell dilution series, top-read. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. The FlexStation 3 reader combines Molecular Devices SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. XML file. The plate was mixed gently for 2 minutes on a plate shaker and then allowed to incubate at room temperature for 10 minutes to stabilize luminescence. #6590-0118) Filter 665/10 (Molecular Devices cat. The list is a standard grouping supplied by Molecular Devices and includes some which cannot be used on this instrument; they can be deleted. Optimizing settings in Standard 384 well black plate A standard black 384 well plate was set up as described in above Table 4. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. Filter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. x. Contact Us Phone: +1-800-635-5577 Web: Email: [email protected] nM Z Factor at EC. Tunable wavelength selection / monochromator. Plus 384. 4 Equipment. 2 OD at 200 nm; this decreased to 0. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. Supplier: Molecular Devices. flat meniscus), but is approx 15 mL. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. For a higher . UV and visible absorbanceLocate the User Guide on your SoftMax Pro computer for version 6 and version 7. Data for a 384-well plate can be collected in less than two minutes, as opposed to up to 30Molecular Devices Corporation (the “Seller”) shall not be liable for any incidental or consequential damages. Molecular Devices deliver high throughput while showing good correlation with manual patch clamping data. Can read 96 well plates (optional 384 well). Page 1 of 8 . 384 only) Selects either 96-well or 384-well plate for a stand-alone read. There is no product in the shopping cart, buy it!. Spectrophotometer 또는 Microplate Reader 상의 260nm에서 측정된 DNA 샘플의 흡광도는…. SpectraMax® Paradigm Plate Reader Oksana Sirenko, Cathy Olsen, Vanessa Ott and Evan F Cromwell Molecular Devices, LLC, 1311 Orleans Drive, Sunnyvale, CA 94089 There has been a trend in the past decade from biochemical assays toward cell based assays. Here, we show that Molecular Devices microplate readers can easily and noninvasively measure fluorescent proteins in living cells. SpectraMax ABS Plus Microplate Reader (Molecular Devices cat. 375 pM 1 FP Fluorescence Polarization detection for fluorescein- or rhodamine-like labels Using specific LED and EX/EM filters for 6- to 1536-well plates 0200-7009POS 0200-7010POS Fluorescein FP EX 485 nm, EM 535P and 535S nm Rhodamine FP EX 535 nm, EM 595P and 595S nm 96–1. All stand-alone instrument functions can be controlled using the software. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. Unmatched throughput FLIPR Membrane Potential Assay Kits allow the elimination of wash steps and much shorter read times on the instrument. 20 plates/hour (2-minute read time) 96-well 1,920 data points/hour 384-well 7,680 data points/hour 1536-well 30,720 data points/hour Bar code manual mode Keyboard entry Bar code remote mode Short or long side Temperature control Ambient +5°C to 40°C General specifications Dimensions (in. Outer wells aligned for proper viewing with FLIPR. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Assay setup for a 384-well low-volume plate. 384-Well SBS Microplate, clear, square well, flat bottom, sterile, with cover, working volume 90 µl, packaged in sleeves of 10. A ten-fold serial dilution of purified luciferase in PBS with 0. 312 gain calibration failed. The modular platform allows upgrading to expand functionality as your laboratory workflows change. Abs). SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. 046 to 0. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Two high-energy xenon flash lamps: Reading capabilities. the Molecular Devices SPECTRAmax® 190 and 250 plate. Published Dec 16, 2020 | Updated Dec 16, 2020. Plate Formats: 6- to 384-well plates. Pre-read measurements were made with 200 µL water in each well of a Costar UV plate, after which the plate was emptied and blotted dry. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Following the flushing of the machine as described in Section 3. ©2017 Molecular Devices, LLC . An industry-renowned instrument for monitoring GPCRs and ion channels. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96- or 384-well head. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, andSpectraMax® iD5 Multi-Mode Microplate Readers,6 to 384-Well Microplates, Molecular Devices, LLC (CAT#: STEM-LE-0504-LC) Product Keyword.